FIGURE

Fig. 1

ID

ZDB-FIG-140702-30

Publication

van der Vaart et al., 2014 - The DNA Damage-Regulated Autophagy Modulator DRAM1 Links Mycobacterial Recognition via TLR-MYD88 to Autophagic Defense

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Fig. 1

Stress-Induced Autophagy Is Not Beneficial for Defense of Zebrafish Embryos against Mycobacterial Infection

(A) Schematic representation of the effects of 3-MA, Cq, and Ar-12 on autophagosome formation and autophagic flux.

(B) GFP-Lc3 embryos 2 dpf were treated for 24 hr with DMSO (control), 3-MA, Cq, or Ar-12. Representative confocal micrographs of endothelial cells at 3 dpf are shown.

(C) GFP-Lc3 embryos treated as described for (A) injected with Mm. Representative confocal micrographs of infected cells at 3 dpf are shown. Boxed areas are detailed below; arrowheads indicate overlap between Mm and Lc3.

(D) AB/Tupfel long fin (AB/TL) embryos treated as described for (A) infected with Mm. Bacterial pixel counts were determined at 3 dpi.

Data (mean ± SEM) is pooled from at least two individual experiments (n e 50 embryos per group). See also Figure S1.

Expression Data

Gene:	EGFP
Fish:	zf155Tg
Conditions:	chemical treatment: pharmaceutical bacterial treatment: Mycobacterium marinum, chemical treatment: 3-methyladenine bacterial treatment: Mycobacterium marinum, chemical treatment: OSU-03012 bacterial treatment: Mycobacterium marinum, chemical treatment: chloroquine bacterial treatment: Mycobacterium marinum
Anatomical Term:	endothelial cell autophagosome
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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