FIGURE

Fig. 5

ID

ZDB-FIG-140623-23

Publication

O'Brien et al., 2014 - MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

miR30a knockdown leads to abnormal somite and myosin fiber morphology, and to increased Six1 expression. Live images, anterior to the left, at (A) 48 hpf (control, n = 54/58; miR30a MO, n = 49/64; six1a mRNA, n = 36/45) and (B) 72 hpf (control, n = 75/75; miR30a MO, n = 30/40; six1a mRNA, n = 28/33) showing that miR30a knockdown (middle row) disrupts normal somite morphology to phenocopy six1a mRNA overexpression (bottom row). (C) Confocal images of MyHC stained with the A4.1025 antibody (control, n = 23/25; miR30a MO, n = 19/28; six1a mRNA, n = 11/20), and (D) loss of birefringence [mean±s.e.m.; non-injected control (NIC) n = 37 total embryos; control MO, n = 23; miR30a, n = 56; *P = 0.027, ANOVA with Bonferroni′s post-hoc test] demonstrate abnormal muscle fiber morphology upon miR30a loss at 48 hpf. (E,F) Increasing concentrations of miR30a-targeted MO result in a dose-dependent increase in (E) Six1 protein expression as visualized by western blot analysis at 48 hpf and (F) quantified following normalization to GAPDH. Scale bars: 100µm. For A, B, n = the number of embryos represented by each image/the total number of embryos analyzed.

Expression Data

Antibody:	Ab-A4.1025
Fish:	WT WT + MO3-mir30a
Knockdown Reagent:	MO3-mir30a
Anatomical Term:	muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-mir30a
Knockdown Reagent:	MO3-mir30a
Observed In:	skeletal muscle cell somite
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.