FIGURE

Fig. S4

ID

ZDB-FIG-140624-10

Publication

O'Brien et al., 2014 - MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

microRNA-30a targeting morpholino specificity confirmed by in situ hybridization and immunoblot analyses. In situ hybridization (ISH) analysis (A, control: n=17/17 total embryos, miR30a MO: n=14/18) for microRNA-30a (miR30a) at 48 hours post fertilization (hpf) reveals a decreased expression of miR30a upon injection of miR30a-targeting morpholino (MO). Transverse cross-sections at 18 µm of 48 hpf embryos stained by ISH for miR30a (B) confirm miR30a is expressed in the somites (S), (NT=neural tube, NC=notochord). Western blot analysis (C) quantified at 48 hpf following normalization to GAPDH demonstrates that miR30a-targeted MO injection into 1-4 cell stage embryos leads to an increase in Six1 protein expression, which is lost if miR30a is co-injected with miR30a MO. Further, six1a mRNA injection does result in increased levels of Six1 protein expression.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.