FIGURE

Fig. 3

ID

ZDB-FIG-140623-21

Publication

O'Brien et al., 2014 - MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1

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Fig. 3

miR30a overexpression functionally phenocopies six1a/b knockdown. Overexpression of miR30a leads to abnormal somite morphology as observed in (A) phalloidin-stained embryos (control, n = 42/49; miR30a, n = 46/77) at 24hpf, and confocal images of (B) both fast and slow MyHC with A4.1025 antibody (arrows indicate crossover of fibers) and (C) fast-muscle specific fibers with F310 antibody (the inset demonstrates a decrease in cross-sectional diameter of the trunk muscle) at 24 hpf. (D) Polarized-light-mediated detection of birefringence of embryos at 48 hpf revealed disarrayed muscle fiber organization upon miR30a overexpression. (E) A quantification (mean±s.e.m.) of the results shown in D. Non-injected, n = 23 total embryos; control miR, n = 25; miR30a, n = 21. *P = 0.008, ANOVA with Bonferroni′s post-hoc test. (F) A quantified increase in cleaved caspase-3 (CC3) is also observed upon miR30a overexpression at 24 hpf. Non-injected, n = 23 total embryos; control miR (1ng), n = 25; miR30a (0.5ng), n = 20. *P<0.05, ANOVA with Bonferroni′s post-hoc test. Scale bars: 50µm. For A, n = the number of embryos represented by each image/the total number of embryos analyzed.

Expression Data

Antibodies:	Ab-A4.1025 Ab-F310
Fish:	WT
Anatomical Terms:	fast muscle cell muscle cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.