FIGURE

Fig. S1

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ZDB-FIG-140624-8

Publication

O'Brien et al., 2014 - MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1

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Fig. S1

Rescue of six1a morphant phenotypes is observed with overexpression of six1a mRNA. Immunofluorescent (IF) staining for multiple SIX family members at 24 hours post-fertilization (hpf) reveals a decrease in protein expression in the somites when six1a morpholino (MO) is injected in 1-4 cell embryos (A). Injection of sixla MO leads to downregulation of Six1 protein specifically as observed by Western blot analysis (B); with this protein expression rescued by co-injection of six1a mRNA with six1a MO. Quantification of cleaved-caspase-3 IF staining (C) at 24 hpf demonstrates that cell survival is also rescued with six1a mRNA overexpression in six1a morphants, ***p<0.0001, ANOVA with Bonferroni’s post-hoc test. Live images (D) at 24 hpf, anterior to the left, show that the U-shaped somite morphology observed in six1a morphants is rescued to normal V-shape with six1a mRNA overexpression (control: n=13/13, 13 ng six1a MO: n=8/10, 13 ng six1a MO + 100pg six1a mRNA: n=12/17). Inset images confirm stage-matching across groups.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.