FIGURE

Fig. 9

ID

ZDB-FIG-140506-41

Publication

Zhao et al., 2014 - Tissue specific roles for the ribosome biogenesis factor Wdr43 in zebrafish development

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Fig. 9

Inhibition of p53 signaling can partially rescue fan mutant craniofacial defects.

(A–B) Whole mount IHC analysis of p53 expression in wild type (A) and fan mutants (B). (C, D) qRT-PCR analysis of d113p53 (C) and mdm2 (D) gene in wild type and fan mutants at 24, 48 and 72 hpf. (E–G) Bright field images of live 3 dpf wild type (E), fan mutant (F), and fan mutant injected with p53MO (G). (H) Percent zebrafish exhibiting normal eye development in uninjected (Uninj), control MO (CMO), and p53MO injected embryos. (I–K) TUNEL stained and sectioned wild type (WT) (I), fan mutant (J), and fan mutant injected with p53 MO (K). Arrows indicate apoptotic cells. (L) Statistical analyses revealed significantly reduced apoptosis in fan mutants injected with p53MO. (P value<0.01).

Expression Data

Genes:	mdm2 tp53
Fish:	WT wdr43^{tft001/tft001}
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wdr43^{tft001/tft001} wdr43^{tft001/tft001} + MO5-tp53
Knockdown Reagent:	MO5-tp53
Observed In:	apoptotic process central nervous system morphogenesis embryonic camera-type eye morphogenesis embryonic viscerocranium morphogenesis eye pharyngeal arch signal transduction by p53 class mediator
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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