ZFIN Image: Zhao et al., 2014, Fig. 9

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Figure Caption

Fig. 9

Inhibition of p53 signaling can partially rescue fan mutant craniofacial defects.

(A–B) Whole mount IHC analysis of p53 expression in wild type (A) and fan mutants (B). (C, D) qRT-PCR analysis of d113p53 (C) and mdm2 (D) gene in wild type and fan mutants at 24, 48 and 72 hpf. (E–G) Bright field images of live 3 dpf wild type (E), fan mutant (F), and fan mutant injected with p53MO (G). (H) Percent zebrafish exhibiting normal eye development in uninjected (Uninj), control MO (CMO), and p53MO injected embryos. (I–K) TUNEL stained and sectioned wild type (WT) (I), fan mutant (J), and fan mutant injected with p53 MO (K). Arrows indicate apoptotic cells. (L) Statistical analyses revealed significantly reduced apoptosis in fan mutants injected with p53MO. (P value<0.01).

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Acknowledgments

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