FIGURE

Fig. 8

ID

ZDB-FIG-140425-15

Publication

Han et al., 2014 - The Nogo-C2/Nogo receptor complex regulates the morphogenesis of zebrafish lateral line primordium through modulating the expression of dkk1b, a Wnt signal inhibitor

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Fig. 8

Morphants of p75, TROY, and Nogo-C2/NgRH1a with malformed PLL primordium exhibit decreased expression of downstream markers of Fgf signaling.

Whole-mount in situ hybridization was performed with probes against markers of PLL primordium organization in control embryo and the indicated morphants. The expression patterns of cxcr4b (A–D), cxcr7b (E–H), and lef1 (I–L) were similar in control embryo and morphants. Expression of downstream markers of Fgf signaling (pea3 (M–P), dkk1b (Q–T), and atoh1a (U–X)) were reduced in all morphants examined as compared to the control. (α–δ) The expression pattern of fgfr1a in the PLL primordium was no different between the control and the morphants. Scale bar, 20 μm.

Expression Data

Genes:	ackr3b atoh1a cxcr4b dkk1b etv4 fgfr1a lef1
Fish:	WT WT + MO1-ngfrb WT + MO3-tnfrsf19 WT + MO1-rtn4rl2a + MO7-rtn4a
Knockdown Reagents:	MO1-ngfrb MO1-rtn4rl2a MO3-tnfrsf19 MO7-rtn4a
Anatomical Term:	posterior lateral line primordium
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ngfrb WT + MO3-tnfrsf19 WT + MO1-rtn4rl2a + MO7-rtn4a
Knockdown Reagents:	MO1-ngfrb MO1-rtn4rl2a MO3-tnfrsf19 MO7-rtn4a
Observed In:	posterior lateral line primordium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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