FIGURE

Fig. S1

ID

ZDB-FIG-140305-52

Publication

Thakur et al., 2014 - Dysregulated phosphatidylinositol signaling promotes endoplasmic reticulum stress-mediated intestinal mucosal injury and inflammation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

The cdipt^lop (lop) and cdipt^hi559 (hi559) mutants exhibit identical GI phenotype and fail to rescue each other in complementation assay. (A) Lateral view of ISH of intestine-specific markers vil1 and anxa2b showing reduced expression in the hi559 intestine at 5-dpf. (B-F) Morphology of intestine shown by bright-field images (left panel) and fluorescent images (right panel, Nile-Red staining shown by white outline). (B) GI morphology of hi559 larvae at 6-dpf shows smaller intestine and lumen. (C) GI morphology of lop larvae shows smaller intestine and lumen at 8-dpf comparable to the 6-dpf hi559 larvae. (D) Cdipt^hi559/lop (hi559 X lop) trans-heterozygote mutant larvae show smaller intestinal lumen at 7.5-dpf comparable to the 6- dpf hi559 larvae. (E) Wild-type embryos were microinjected with either control morpholino or splice-blocking morpholino against cdipt at 1-cell stage and larvae were analyzed at 6-dpf (Thakur et al., 2011). Cdipt morphants show smaller intestinal structures replicating hi559 phenotype. (F) Wild-type larvae were treated with PI synthase inhibitor δ-HCH shows smaller intestinal structures compared to DMSO treated larvae.

Expression Data

Genes:	anxa2b vil1
Fish:	WT cdipt^{hi559Tg/hi559Tg}
Anatomical Term:	intestine
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT cdipt^{hi559Tg/hi559Tg} cdipt^nt9/nt9 WT + MO5-cdipt cdipt^nt9/+; cdipt^hi559Tg/+
Condition:	chemical treatment: delta-hexachlorocyclohexane
Knockdown Reagent:	MO5-cdipt
Observed In:	intestine
Stage Range:	Day 6 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.