FIGURE

Fig. 5

ID

ZDB-FIG-140305-48

Publication

Thakur et al., 2014 - Dysregulated phosphatidylinositol signaling promotes endoplasmic reticulum stress-mediated intestinal mucosal injury and inflammation in zebrafish

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Fig. 5

Intestinal pathologies of tunicamycin-treated wild-type larvae. (A) ISH with fabp2 shows smaller intestine in tunicamycin (TN)-treated larvae. (B) Confocal projections of Tg(fabp2:egfp) intestine (white outline) showing disrupted intestinal architecture of tunicamycin-treated larvae. (C) H&E-stained sections shows abnormal GCs (arrows) and increased luminal bacteria (arrowheads) in tunicamycin-treated larvae. (D) Bar charts showing reduced gut size, GC depletion and increased intestinal bacteria in tunicamycin-treated larvae (n≥10). (E) Confocal projection of Tg(lyzc:egfp) larval intestine shows increased macrophage aggregation (arrows) in tunicamycin-treated larvae (n=15). (F) Confocal projection of Tg(lc3:gfp) intestine shows increased autophagosomes (arrows) in tunicamycin-treated larvae (n=7). *P<0.05, **P<0.01, ***P<0.001. Scale bars: 100 µm (B,E); 20 µm (C); 5 µm (F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT nz117Tg zf155Tg
Condition:	chemical treatment: tunicamycin
Observed In:	esophageal epithelium goblet cell goblet cell intestinal epithelial cell autophagosome intestinal epithelial cell autophagy intestinal epithelial cell positive regulation of autophagy in response to ER overload intestinal epithelium goblet cell intestine intestine leukocyte macrophage response to bacterium
Stage:	Day 6

Phenotype Detail

Acknowledgments

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