FIGURE

Fig. S5

ID

ZDB-FIG-140310-7

Publication

Thakur et al., 2014 - Dysregulated phosphatidylinositol signaling promotes endoplasmic reticulum stress-mediated intestinal mucosal injury and inflammation in zebrafish

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Fig. S5

Unresolved ER stress pathology of hi559 GI tissues. (A-B) ISH shows elevated expression of the crucial ER stress-UPR markers hspa5 (A) and xbp1 (B) in the hi559 GI tissue. (C) RT-PCR showing activation of xbp1 by increased transcription of unspliced (xbp1-u) and spliced xbp1 (xbp1-s) in 5-dpf hi559 GI tissues. β-actin was used as RT-PCR control. (D-E) TEM images of 6-dpf wild-type (left panels) and hi559 pancreatic cells (right panels). (D) Ultrastructure of the secretory granulescontaining endocrine pancreatic cells shows increased ER luminal expansion and vacuolization, multi-lamellar autophagic bodies (red outline), and increased mitophagy (red arrow) in hi559. (E) ER luminal expansion is also apparent in the hi559 pancreatic acinar cells containing zymogen granules. Er: endoplasmic reticulum; Sg, secretory granules; Zm; zymogen granules; Mt, mitochondria. Scale bars: D-E, 500 nm.

Expression Data

Genes:	hspa5 xbp1
Fish:	WT cdipt^{hi559Tg/hi559Tg}
Anatomical Terms:	intestine whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdipt^{hi559Tg/hi559Tg}
Observed In:	intestinal epithelial cell autophagosome intestinal epithelial cell autophagy intestinal epithelial cell autophagy of mitochondrion intestinal epithelial cell endoplasmic reticulum lumen intestinal epithelial cell positive regulation of autophagy in response to ER overload pancreatic acinar cell endoplasmic reticulum lumen
Stage:	Day 6

Phenotype Detail

Acknowledgments

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