ZFIN Image: Thakur et al., 2014, Fig. 5

Image

Figure Caption

Fig. 5

Intestinal pathologies of tunicamycin-treated wild-type larvae. (A) ISH with fabp2 shows smaller intestine in tunicamycin (TN)-treated larvae. (B) Confocal projections of Tg(fabp2:egfp) intestine (white outline) showing disrupted intestinal architecture of tunicamycin-treated larvae. (C) H&E-stained sections shows abnormal GCs (arrows) and increased luminal bacteria (arrowheads) in tunicamycin-treated larvae. (D) Bar charts showing reduced gut size, GC depletion and increased intestinal bacteria in tunicamycin-treated larvae (n≥10). (E) Confocal projection of Tg(lyzc:egfp) larval intestine shows increased macrophage aggregation (arrows) in tunicamycin-treated larvae (n=15). (F) Confocal projection of Tg(lc3:gfp) intestine shows increased autophagosomes (arrows) in tunicamycin-treated larvae (n=7). *P<0.05, **P<0.01, ***P<0.001. Scale bars: 100 µm (B,E); 20 µm (C); 5 µm (F).

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.