Fig. S2

The development of tissues derived from mesoderm and epithelia in the eif3ba mutant. (A-R) Analyses of the development of tissues derived from mesoderm. We examined the development of blood cells (A-B, G-H, M-N) by hbae3 in situ hybridization, blood vessels (C-D, I-J, O-P) on the background of the Tg(flk:EGFP) transgenic fish line, and muscle (E-F, K-L, Q-R) by myhz in situ hybridization, at different stages in wild-type and mutant embryos. From 24 hpf (A-D) to 28 hpf (G-L), no differences are detectable in any of these tissues between the mutants and the wild-type embryos. At 36 hpf (M-R), the blood cells become fewer, the growth of the blood vessels is incomplete at the tail (blue arrows), and the muscle is hypotrophic in the mutants compared with the wild-type embryos. (S-V) Analyses of the development of tissues derived from epithelia. We examined the facial epithelia by tuba in situ hybridization (S-T) and pan-cadherin immuno-staining (U-V) at 24 hpf, and found no differences between the wild-type and eif3ba mutants. (A-T) Lateral view with anterior to the left and dorsal up. WT, wild type; Mut, eif3ba mutant.