FIGURE

Fig. 8

ID

ZDB-FIG-131015-53

Publication

Xia et al., 2013 - Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish

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Fig. 8

TUNEL assay and genetic interaction between eif3ba and p53. (A)–(B′′). Double-labeling with TUNEL (A, B) and anti-EGFP immunofluorescence staining (A′)–(B′) on the background of the Et(gata2a:EGFP)^pku418 line at 22 ss. The neural crest lineage in the cranial region is undergoing apoptosis in the eif3ba mutant (A′′)–(B′′). (C) Western blot result shows the up-regulation of p53 in the eif3ba mutants compared with their normal siblings (Control). (D)–(F′) In the p53^-/- background, the crestin expression is recovered in the cranial region (red triangles) and branchial primordia (red arrows) in the eif3ba mutant embryos at 24 hpf. (G) Efficiency and effect of EIF3B knockdown by RNA interference in HeLa cells. The abundance of EIF3B mRNA is remarkably reduced after siRNA transfection, as shown by real-time RT-PCR (p<0.01, #1836). (H) Western blot detection of p53 expression in EIF3B-siRNA-treated HeLa cells. p53 is up-regulated in the treated group compared with the controls. WT, wild type; Mut, eif3ba mutant. (G, H) Mock: cells were transfected without any RNA; Con.si: cells were transfected with negative control RNA; #1836: cells were transfected with #1836 siRNA against human EIF3B. (A–B′′, D–F) Lateral view with anterior to the left and dorsal up; (D′)–(F′) dorsal view with anterior to the left.

Expression Data

Gene:	crestin
Fish:	eif3ba^{la024215Tg/la024215Tg} WT + MO4-tp53 eif3ba^{la024215Tg/la024215Tg} + MO4-tp53
Knockdown Reagent:	MO4-tp53
Anatomical Term:	pharyngeal arch 3-7 neural crest cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eif3ba^{la024215Tg/la024215Tg}
Observed In:	cranial neural crest cell
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(1), Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S., and Zhang, B., Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish, 83-96, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.