FIGURE

Fig. 5

ID

ZDB-FIG-131015-44

Publication

Xia et al., 2013 - Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish

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Fig. 5

Tracing the migration of cranial NCCs in Et(gata2a:EGFP)^pku418 fish line during early developmental stages from 19 ss to 25 ss. (A)–(L) The dynamic EGFP expression pattern revealed by confocal microscopy of the head (A, E, I), trunk (B, C, F, G, J, K) and heart (D, H, L) regions of the Et(gata2a:EGFP)^pku418 fish line. (A, B) Pre-migratory NCCs in the head (white triangle) and the anterior trunk (white arrow) of 19 ss embryos. (C) No EGFP-positive cells are present in the tail at 19 ss. (D) EGFP-positive cells reach the primary heart field (red arrows). (E)–(G) Pre-migratory and migrating NCCs in the head (white triangle) and the anterior trunk (white arrow) of 21 ss embryos. Cranial NCC-derived tissues, including some brain neurons (yellow triangles) and some non-NCC-derived, but also nrp2b-expressing tissues, including cells in the gut endoderm (yellow asterisks) and spinal cord neurons (red asterisk), are all labeled by the green fluorescence signal. (H) By 21 ss, the bilateral myocardium precursors merge to form one intact heart, and EGFP-positive cells are clearly detected in the primordial heart (red arrow) and branchial arches (red triangle). (I)–(L) Migrating NCCs in the head (white triangle) and the anterior trunk (white arrow) of 25 ss embryos. Some pigment cells are labeled by the fluorescence signal (yellow arrow) in addition to other EGFP-positive tissues. (A′)–(L′) Bright field images merged with the corresponding fluorescent images from (A)–(L).

Expression Data

Gene:	EGFP
Fish:	nrp2b^pku418Et
Anatomical Terms:	brain CNS neuron (sensu Vertebrata) gut endoderm head cranial neural crest cell heart primordium pharyngeal arch 3-7 pigment cell (sensu Vertebrata) spinal cord neural tube neuron trunk anterior region trunk neural crest cell
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(1), Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S., and Zhang, B., Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish, 83-96, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.