FIGURE

Fig. 2

ID

ZDB-FIG-131015-41

Publication

Xia et al., 2013 - Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish

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Fig. 2

Abnormal development of cranial NCCs and their derivative tissues in the eif3ba mutant. (A)–(D) The expression of crestin in the mutant compared with the wild-type at 16 ss and 20 ss. While at 16 ss, there is little difference in the staining between wild-type and mutant embryos(A, B), the expression of crestin starts to decrease specifically in the cranial region at 20 ss (C, D, red triangles). (E)–(H) The migration pattern of cranial NCCs in the branchial primordia differs between the mutant and the wild-type embryos as revealed by in situ hybridization. The expressions of both dlx2a (E, F) and nrp2b (G, H) are reduced in the branchial primordia (red arrows) of mutant embryos at 24 hpf. (I)–(L) The PAs (red brackets) did not form in the mutants as revealed by in situ hybridization of vgll2a at 48 hpf and sox9a at 72 hpf. (M)–(P) At 72 hpf, the facial cartilage structure is revealed by sox9a in situ hybridization (M, N) or Alcian blue staining (O, P). WT, wild type; Mut, eif3ba mutant. (A–D, I, J) Lateral view with anterior to the left and dorsal up; (E–H, M–P) dorsal view with anterior to the left; (I′, J′) ventral view with anterior to the left.

Expression Data

Genes:	crestin dlx2a nrp2b sox9a vgll2a
Fish:	WT eif3ba^{la024215Tg/la024215Tg}
Anatomical Terms:	head pharyngeal arch 3-7 pharyngeal arch 3-7 neural crest cell
Stage Range:	20-25 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	eif3ba^{la024215Tg/la024215Tg}
Observed In:	cranial neural crest neural crest cell development pharyngeal arch whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 381(1), Xia, Z., Tong, X., Liang, F., Zhang, Y., Kuok, C., Zhang, Y., Liu, X., Zhu, Z., Lin, S., and Zhang, B., Eif3ba regulates cranial neural crest development by modulating p53 in zebrafish, 83-96, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.