FIGURE

Fig. 6

ID

ZDB-FIG-131211-38

Publication

Phng et al., 2013 - Filopodia are dispensable for endothelial tip cell guidance

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Endothelial tip cell selection and ectopic sprouting occur in the absence of filopodia. (A-F) Tg(Kdr-l:ras-Cherry)^s916 zebrafish embryos injected with 15 ng control or Dll4 morpholino, treated with either 0.4% DMSO or 0.08 μg/ml Lat. B from 30 hpf and imaged at 49 hpf. (G-O) Tg(Kdr-l:ras-Cherry)^s916 embryos injected with HSP:Vegfa165-IRES-nlsEGFP plasmid. (G-I′,M) Uninjected and injected embryos were heat shocked at 24 hpf, treated with 0.4% DMSO or 0.08 μg/ml Lat. B from 29 hpf, and examined at 47 hpf. (J-L′,N,O) Uninjected and injected embryos were heat shocked at 51 hpf, treated with 0.4% DMSO or 0.1 μg/ml Lat. B at 52 hpf, and examined at 76 hpf. Arrowheads indicate cells expressing nuclear EGFP and Vegfa165. Arrows highlight filopodia. Yellow arrows indicate ectopic sprout from the dorsal aorta. Asterisk indicates a cell expressing high levels of Vegfa165. DA, dorsal aorta; CV, caudal vein; ISV, intersomitic vessel. Scale bars: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development