FIGURE

Fig. S6

ID

ZDB-FIG-131106-7

Publication

Hess et al., 2012 - Intravital Imaging of Thymopoiesis Reveals Dynamic Lympho-Epithelial Interactions

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Fig. S6

Reduced numbers of ikaros-expressing cells in hematopoietic tissues in cxcl12b morphants

ÿEmbryos double-transgenic for ikaros:eGFP and foxn1:mCherry were injected with a cxcl12b-specific anti-sense morpholino and assayed at 63 hpf for green cells in the AGM (aorta-gonad-mesonephros and CHT (caudal hematopoietic tissue) regions. Photographs representative of ~250 wild-type and 48 mutant embryos. Quantitative assessment (values shown are mean ± s.e.m.) revealed significant (Fisher´s exact probability test) reductions in both types of tissues. AGM: wild-type fish exhibit 8±0.58 (n=3) and cxcl12b morphants exhibit 0.75±0.25 (n=4) ikaros+ cells per high-power field (P<0.001); CHT: wild-type fish exhibit 32±3.5 (n=3) and cxcl12b morphants exhibit 8.5±1.3 (n=6) ikaros⁺ cells per high-power field (P<0.001). Anatomical landmarks are indicated: PCV, posterior cardinal vein; CV, caudal vein; CA, caudal artery; DA, dorsal aorta. Scale bars, 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fr101Tg; fr103Tg + MO2-cxcl12b
Knockdown Reagent:	MO2-cxcl12b
Observed In:	ventral wall of dorsal aorta lymphocyte
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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Reprinted from Immunity, 36(2), Hess, I., and Boehm, T., Intravital Imaging of Thymopoiesis Reveals Dynamic Lympho-Epithelial Interactions, 298-309, Copyright (2012) with permission from Elsevier. Full text @ Immunity