Fig. S5

Chemokines regulate thymus homing

Double-transgenic fish (ikaros:eGFP [green fluorescence]; foxn1:mCherry [red fluorescence]) were injected with gene-specific anti-sense morpholinos singly or in combination. The type of gene-specific morphant is indicated at the top of panels in the first column. The cxcl12b morphant exhibits a pre-thymic defect. See also Figure S6.
Shown are still photographs from movies taken at several different time points, beginning at 52 hpf (00hours:00minutes; first column). The panels of the latest time point (fourth column) contain insets depicting enlargements of the thymic (white dotted line) and parathymic regions. At 63 hpf, the numbers of cells present in the thymic rudiments (marked with circles) was determined and are presented in the third column. The presence of e5 cells per rudiment was considered to be indicative of normal colonization; hence, for statistical purposes, the fraction of embryos with 0-4 intrathymic cells was compared to those containing e5 cells: P<8.32 x 10^-10; ccl25b morphant, P<0.256; cxcl12b morphant, P<2.21x10^-9; ccl25b and cxcl12b double-morphant, P<3.21x10^-6.; ccl25a and cxcl12b double-morphant, P<7.62x10^-8; Fisher´s exact probability test. The numbers of embryos analysed for each group are indicated. 50μm for overviews, 10μm for high-power views (insets). The numbers of embryos that were subjected to the analyses are shown in each panel.