FIGURE

Fig. 3

ID

ZDB-FIG-130426-17

Publication

Choe et al., 2013 - Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation

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Fig. 3

Roles of Wnt Signaling Components in Pouch and CB Cartilage Development(A–L) Alcama immunohistochemistry (green) shows defects in pouches in mutant, fzd8a-MO, and transgenic embryos. Identifiable pouches are numbered. UAS transgenic embryos (capitalized) were doubly positive for nkx2.3:Gal4VP16. Scale bar, 40 μM.(M–X) Whole-mount views of dissected facial cartilages. Arrows indicated fused or abnormal CB cartilages. Arrowhead indicates abnormal hyoid cartilage in fzd8a-MO embryos.(Y–AA) Superimposition of initial (blue) and final (red) still images from time-lapse recordings of fifth pouch development in wild-type, wnt11r^-/-, and wnt4a^-/- embryos. Colored lines indicate cell tracks (shown also below merged images), with filled circles denoting final positions.(AB) Average pouch cell speed in wild-types and mutants. Data represent mean ± SEM and p values are shown for each comparison.See also Figures S1 and S2 and Movies S3, S4, and S5.

Expression Data

Gene:	alcama
Antibody:	zn-8
Fish:	WT wnt4^fh295/fh295 wnt11^fh224/fh224 WT + MO2-fzd8a el48Tg; el93Tg el190Tg; el93Tg el262Tg; el93Tg el268Tg; el93Tg el320Tg; el93Tg el322Tg; el93Tg el421Tg; el93Tg wnt11^fh224/fh224; wnt4^fh295/fh295
Knockdown Reagent:	MO2-fzd8a
Anatomical Term:	pharyngeal pouch cell-cell junction
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt4^fh295/fh295 wnt11^fh224/fh224 WT + MO2-fzd8a el48Tg; el93Tg el190Tg; el93Tg el262Tg; el93Tg el268Tg; el93Tg el320Tg; el93Tg el322Tg; el93Tg el421Tg; el93Tg wnt11^fh224/fh224; wnt4^fh295/fh295
Knockdown Reagent:	MO2-fzd8a
Observed In:	ceratobranchial cartilage embryonic viscerocranium morphogenesis pharyngeal pouch pharyngeal pouch cell migration whole organism
Stage Range:	Prim-15 to Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 24(3), Choe, C.P., Collazo, A., Trinh, L.A., Pan, L., Moens, C.B., and Crump, J.G., Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation, 296-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell