Fig. 3

Distinct, Lineage-Restricted Defects in Hematopoiesis in rpl22 and rpl22l1 Morphants (A and B) WISH analysis of thymocyte development with the indicated probes in rpl22 (A) and rpl22l1 morphants (B) at 5 dpf. Thymus, red dashed rectangles. (C–E) Evaluation of thymus colonization and HSC emergence in rpl22l1 and rpl22 morphants. Tg(cd41:EGFP) embryos were injected with Rpl22l1 MO, Rpl22 MO, or MM control, after which seeding of the thymus and HSC emergence was assessed at 3.5 dpf by tracking the presence of GFP+ cells in the thymus (red circles) or VDA (between the red and white dashed lines). Emerging HSC are indicated by arrows. The area between the dashed white and yellow lines is the pronephric duct. The number of CD41-EGFP^low HSCs was quantified in six representative embryos per group and presented as mean ± SD. *p < 0.001. Images depict phenotypes representative of at least three separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure 3.