FIGURE

Fig. 4

ID

ZDB-FIG-130409-11

Publication

Zhang et al., 2013 - Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs

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Fig. 4

Rpl22l1 Knockdown Blocks HSC Emergence and Definitive Hematopoiesis (A and B) The effect of Rpl22l1 knockdown on development of definitive erythroid (A; a-e1globin) and myeloid (B; lyc) progenitors was assessed by WISH with the indicated probes at 5 dpf in embryos injected with Rpl22l1 MO or MM control. Developing kidney, boxed area and asterisk; CHT, blue arrowheads; VDA, red arrowheads. (C–E) Effect of Rpl22l1 knockdown on c-myb and runx1 expressing progenitors. Embryos were injected with MO as above and evaluated by WISH at 5 dpf (C), 36 hpf (D), and 24 hpf (E) with the indicated probes. Developing kidney (C; red boxed insert, blue asterisk); thymus (C; red boxed inset, red circle); AGM, (D; arrows); AGM (E; blue boxed area, arrows); and erythro-myeloid progenitors (E; EMP, red boxed inset, red arrowhead). (F) Impact of Rpl22l1 knockdown on the vasculature. Dorsal aorta (efnb2a), vasculature (flk1), and pronephros (cdh17) were assessed by WISH in control and rpl22l1 morphant embryos as above with the indicated probes (arrows). Images depict phenotypes representative of at least three separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure S4.

Expression Data

Genes:	cdh17 efnb2a hbae1.1 kdrl lyz myb runx1
Fish:	WT WT + MO1-rpl22l1
Knockdown Reagent:	MO1-rpl22l1
Anatomical Terms:	common myeloid progenitor dorsal aorta hematopoietic system erythroid progenitor cell kidney common myeloid progenitor kidney erythroid progenitor cell kidney hematopoietic stem cell pronephros thymus hematopoietic stem cell vasculature ventral wall of dorsal aorta common myeloid progenitor ventral wall of dorsal aorta hematopoietic stem cell
Stage Range:	Prim-5 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 24(4), Zhang, Y., Duc, A.C., Rao, S., Sun, X.L., Bilbee, A.N., Rhodes, M., Li, Q., Kappes, D.J., Rhodes, J., and Wiest, D.L., Control of hematopoietic stem cell emergence by antagonistic functions of ribosomal protein paralogs, 411-425, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell