FIGURE

Fig. 6

ID

ZDB-FIG-120831-6

Publication

Delaurier et al., 2012 - Histone deacetylase-4 is required during early cranial neural crest development for generation of the zebrafish palatal skeleton

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Reduction or absence of neural crest cells in MO-injected embryos is associated with palatal defects. (A,C, E) Lateral views of live sox10:EGFP transgenic embryos at 24 hpf. Anterior is towards the left, dorsal is upwards. (B,D,F) Ventral view of Alcian Blue (cartilage) and Alizarin Red (bone) stained palatal skeletons of the same individual fish shown in A,C,E, fixed and flat-mounted at 6 dpf. Anterior is upwards. (A) Uninjected embryos (n = 7) showed GFP expression in neural crest-derived tissues in the head including arch 1 (a1) and arch 2 (a2), and GFP-positive cells populate the anterior-ventral margin of the face where the palatal skeleton forms (indicated by red arrow). (B) Uninjected embryos had normal development of the ethmoid plate (ep) and trabeculae communis (tc) of the palatal skeletons. (C and E) hdac4 MO-injected embryos (n = 6) showed GFP expression in neural crest-derived tissues in the head, including arch 1 (a1) and arch 2 (a2), but a lack of cells in the anterior-ventral margin of the face at 24 hpf (indicated by red arrows) (D and F) The same hdac4 MO-injected embryos in C and E had palatal skeleton defects at 6 dpf (n = 6/6), including defects in formation of the trabeculae communis (D), and ethmoid plate (F). A,C,E scale bars = 100 μm, B,D,F scale bar = 100 μm.

Expression Data

Gene:	EGFP
Fish:	ba2Tg ba2Tg + MO1-hdac4 + MO2-hdac4
Knockdown Reagents:	MO1-hdac4 MO2-hdac4
Anatomical Terms:	cranial neural crest cell head antero-ventral region mandibular arch skeleton pharyngeal arch 2 skeleton
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-hdac4 + MO2-hdac4
Knockdown Reagents:	MO1-hdac4 MO2-hdac4
Observed In:	cranial neural crest antero-ventral region cranial neural crest cell ethmoid cartilage trabecula communis
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.