FIGURE

Fig. 3

ID

ZDB-FIG-120831-3

Publication

Delaurier et al., 2012 - Histone deacetylase-4 is required during early cranial neural crest development for generation of the zebrafish palatal skeleton

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Fig. 3

Over-expression ofhdac4mRNA rescues palatal defects associated with MO-injection and causes midline craniofacial defects. (A-D) Alcian Blue (cartilage) and Alizarin Red (bone) stained embryos and dissected palatal skeletons. (A and B) Ventral view of whole-mount skeletal preparations of 6 dpf larvae, anterior is upwards, ventral is facing. (C and D) Flat-mounts of palatal skeletons at 6 dpf. Anterior is upwards. (A) Uninjected fish have normal development of craniofacial cartilage and bone. (B) hdac4 mRNA injection resulted in cyclopia and a loss of normal midline patterning (indicated by white arrow). (C) Uninjected fish have normal palatal skeletons. (D) hdac4 mRNA injection results in ‘stick’-like palatal skeletons. (E) Co-injection of hdac4 mRNA (20 ng/µl) and MO1 + 2 (12 + 2 mg/ml) resulted in protein levels similar to levels in uninjected embryos 24 hours post-injection, compared with injection of mRNA or MOs alone. (F) Embryos used for the protein assay were also raised to 6 dpf and scored for palatal phenotypes. Co-injection of hdac4 mRNA with MO1 + 2 resulted in a decrease of MO-like defects (i.e. cleft, hole, ethmoid plate (EP) defects) compared with MO-injection alone. A and B, scale bar = 200 μm; C and D, scale bar = 100 μm.

Expression Data

Gene:	hdac4
Antibody:	Ab1-hdac4
Fish:	AB AB + MO1-hdac4 + MO2-hdac4
Knockdown Reagents:	MO1-hdac4 MO2-hdac4
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-hdac4 + MO2-hdac4
Knockdown Reagents:	MO1-hdac4 MO2-hdac4
Observed In:	ethmoid cartilage palate
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.