FIGURE

Fig. 3

ID

ZDB-FIG-120727-37

Publication

Niu et al., 2012 - Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish

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Fig. 3

The sq198 mutation causes loosening of the cell-cell junction in the digestive organs. (A,B) TEM analysis of intestinal epithelium in 5 dpf wildtype and mutant embryos. The epithelial cells are tightly adhered to each other in the wildtype (WT) (A, white arrows) but are separated by clearly visible gaps in the mutant (mu) (B, white arrows). (C–H) Double immunofluorescence staining using the antibodies against the basement component Laminin (red) and the ER marker PDI (green). In the wild type control at 5 dpf, Laminin forms the normal lining along the basement layer (C) that nicely separates the liver and intestine (yellow arrows) (G). No apparent accumulation of Laminin in the ER (E,G). In contrast, the mutant did not form an apparent basement layer to separate the liver and intestine (white arrowheads) (D,H) and the mutant cells accumulated Laminin in the ER (white arrowheads) (D,F,H).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sec13^sq198/sq198 (AB)
Observed In:	intestinal epithelium cell junction liver basement membrane
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.