Fig. 3

Fig. 3 The sq198 mutation causes loosening of the cell-cell junction in the digestive organs. (A,B) TEM analysis of intestinal epithelium in 5 dpf wildtype and mutant embryos. The epithelial cells are tightly adhered to each other in the wildtype (WT) (A, white arrows) but are separated by clearly visible gaps in the mutant (mu) (B, white arrows). (C–H) Double immunofluorescence staining using the antibodies against the basement component Laminin (red) and the ER marker PDI (green). In the wild type control at 5 dpf, Laminin forms the normal lining along the basement layer (C) that nicely separates the liver and intestine (yellow arrows) (G). No apparent accumulation of Laminin in the ER (E,G). In contrast, the mutant did not form an apparent basement layer to separate the liver and intestine (white arrowheads) (D,H) and the mutant cells accumulated Laminin in the ER (white arrowheads) (D,F,H).