FIGURE

Fig. 4

ID

ZDB-FIG-120727-38

Publication

Niu et al., 2012 - Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

The sq198 mutation impairs the protein secretory pathway. (A,A2,B,B2) TEM analysis. Compared to wildtype (WT), the mutant (mu) exhibited disorganized chondrocytes (compare A and B) and less-accumulated ECM (compare A′ and B′). A′ and B′ represent high resolution pictures of boxed regions in A and B, respectively. e: ECM; green asterisk: unknown vesicles in a mutant chondrocyte. (C–H) Double immunostaning of the ER resident marker PDI and the secretory protein Collagen II (Col2a) in chondrocytes in 5 dpf wildtype (WT) (C–E) and mutant (mu) (F–H) embryos. (C,F) Immunostaining of PDI (green). (E,H) Immunostaining of Col2a secretion (red). (D,G) Superimpose of PDI and Col2a staining accordingly.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sec13^sq198/sq198 (AB)
Observed In:	chondrocyte chondrocyte cytoplasm chondrocyte extracellular matrix chondrocyte vacuole extracellular matrix constituent secretion
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 367(2), Niu, X., Gao, C., Jan Lo, L., Luo, Y., Meng, C., Hong, J., Hong, W., and Peng, J., Sec13 safeguards the integrity of the endoplasmic reticulum and organogenesis of the digestive system in zebrafish, 197-207, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.