FIGURE

Fig. 4

ID

ZDB-FIG-120725-12

Publication

Sieger et al., 2012 - Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia

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Fig. 4

Microglial Movement Is Controlled via P2Y12 Activation(A) Microglial cells express a P2Y12-GFP fusion protein under the endogenous P2Y12 promoter (p2y12::P2Y12-GFP).(C) Overlay of (A) and (B).(D–F) microglia (pU1::Gal4-UAS-GFP) and neurons (NBT::DsRed) in wild-type (D), Duox-morpholino-injected (E), and P2Y12-morpholino-injected embryos (F) 120 min after central injury. The sites of injury are marked with white arrowheads.(G) Percentage of microglia in the optic tectum moving to the injury site in control (n = 12), DPI-treated (n = 7), Duox morphant (n = 10), MRS2395-treated (n = 7), CBX-treated (n = 12), P2Y12-Mo1-injected (n = 14), P2Y12 Mo2-injected (n = 9), and P2Y12 ctrl Mo-injected (n = 5) brains. Error bars represent standard deviations.(H and I) Dorsal views of a 3 dpf larval brain, showing microglia in the p2y12::P2Y12-GFP line (H) and how P2Y12-morpholino injection strongly decreases P2Y12-GFP expression in these microglia (I).Scale bars for all images: 20 μm. All images were produced using an Olympus FV 1000 with a 40×/NA1.15 objective.See also Figure S2 and Movies S4 and S5.

Expression Data

Genes:	DsRed EGFP GFP TagRFP
Fish:	hdb2Tg; hdb3Tg zf148Tg; zf149Tg zf148Tg; zf149Tg + MO1-duox zf148Tg; zf149Tg + MO1-p2ry12 zf148Tg; zf149Tg + MO2-p2ry12
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO1-duox MO1-p2ry12 MO2-p2ry12
Anatomical Terms:	brain microglial cell brain neuron
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf148Tg; zf149Tg + MO1-p2ry12 zf148Tg; zf149Tg + MO2-p2ry12
Condition:	physical alteration: anatomical structure
Knockdown Reagents:	MO1-p2ry12 MO2-p2ry12
Observed In:	microglial cell activation response to wounding
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 22(6), Sieger, D., Moritz, C., Ziegenhals, T., Prykhozhij, S., and Peri, F., Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia, 1138-1148, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell