FIGURE

Fig. 7

ID

ZDB-FIG-120725-15

Publication

Sieger et al., 2012 - Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Microglial Branching toward Sites of Glutamate Signaling Is Mediated via the Microglial ATP Receptor P2Y12(A) Single-hemisphere injections of caged NMDA. Uncaging leads to Ca²⁺ signaling (beta-actin::GCaMP3.1) in the injected, but not in the control, side (Movie S11, upper). Laser uncaging sites are marked by white arrowheads.(B) Rose plot of microglial response to NMDA uncaging, showing microglia positions before (white) and 30 min after (red) uncaging.(C) Microglial response (pU1::Gal4-UAS-TagRFP) in the same embryo as in (B) 30 min after NMDA uncaging (Movie S11, upper).(D) Single-hemisphere injections of caged NMDA in P2Y12-Mo-injected embryos. Laser uncaging sites are marked by white arrowheads.(E) Rose plot of microglial response to NMDA uncaging in P2Y12-depleted brains showing microglia positions before (white) and 30 min (red) and 60 min (yellow) after uncaging.(F) Microglial response (pU1::Gal4-UAS-TagRFP) in the same embryo as in (E) 60 min after NMDA uncaging (2+, which in turn leads to release of ATP that is sensed by microglia via their P2Y12 receptor.Scale bars for all images: 20 μm. All images were produced using an Andor Spinning Disk Confocal with a 40x/NA1.15 objective.See also Movie S11.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 22(6), Sieger, D., Moritz, C., Ziegenhals, T., Prykhozhij, S., and Peri, F., Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia, 1138-1148, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell