Fig. 5

ATP Does Not Influence Ca²⁺ Wave Formation but Promotes Microglial Movement(A₁) Injection of ATP does not promote Ca²⁺ wave formation (beta-actin::GCaMP3.1).(A₂) Same ATP-injected embryo as in (A₁) shows normal Ca²⁺ wave formation upon laser injury.(B) Ca²⁺ signaling (beta-actin::GCAMP3.1) after IP3 uncaging (Movie S6, left). The location of the uncaging is marked with a red circle.(C) Rose plot of microglial response to IP3 uncaging showing images before uncaging (white) and 7 min (red) and 21 min (yellow) after uncaging.(D) Microglial response (pU1::Gal4-UAS-TagRFP) in the same embryo as in (C) 21 min after IP3 uncaging (Movie S6, right).(E) Ca²⁺ signaling (beta-actin::GCAMP3.1) upon IP3 uncaging in an embryo additionally injected with apyrase (Movie S7, upper left).(F) Rose plot of microglial response to IP3 uncaging in an apyrase-injected brain showing images before (white) and 25 min after (red) uncaging.(G) Microglial response (pU1::Gal4-UAS-TagRFP) in the same embryo as in (F) 25 min after IP3 uncaging (Movie S7, upper right).(H) Ca²⁺ signaling (beta-actin::GCAMP3.1) upon IP3 uncaging in an embryo injected with P2Y12-Mo (Movie S7>, lower left).(I) Rose plot of microglial response to IP3 uncaging in a P2Y12-depleted brain showing microglia positions before (white) and 24 min (red) and 60 min (yellow) after uncaging.(J) Microglial response (pU1::Gal4-UAS-TagRFP) in the same embryo as in (I) 60 min after IP3 uncaging (2+ signaling (beta-actin::GCaMP3.1) upon laser injuries (white arrowheads) in an embryo injected with apyrase.(L) Rose plot of microglial response to laser injury in the apyrase-injected embryo as in (K), showing microglia positions before (white) and 30 min after (red) injury.Scale bars for all images: 20 μm. All images were produced using an Andor Spinning Disk Confocal with a 20×/NA0.7 objective.See also Movies S6, S7, and S8.