FIGURE

Fig. 2

ID

ZDB-FIG-120301-9

Publication

Tam et al., 2012 - Death Receptors DR6 and TROY Regulate Brain Vascular Development

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Fig. 2

Zebrafish Candidate-Based Genetic Screen Identifies Regulators of CNS Angiogenesis and Barriergenesis (A and B) Morpholino-mediated knockdown of BBB-enriched transcripts in Tg(kdrl:egfp) embryos. As described in Experimental Procedures, following a 4 ng morpholino injection, DAPI-positive brain parenchymal nuclei (Parenchymal DAPI; A: right panels) were visually isolated by computationally removing brain vasculature-derived DAPI signal from the DAPI channel. Results representative of at least three independent experiments are shown. Endothelial cells are green, DAPI is blue, rhodamine-dextran is red. MO, morpholino. (C) Quantification of DAPI leakage across the zebrafish BBB. n = 6 (control_MO), n > 6 (DR6_MO, TROY_MO, Spock2_MO, Adcyap1r1_MO, Tspn5_MO, and Tnfrsf1a_MO). Data are presented as the mean ± standard error of the mean (SEM) (^***p < 0.005; ^**p < 0.01; ^*p < 0.05). See also Figure S2.

Expression Data

Gene:	EGFP
Fish:	s843Tg s843Tg + MO1-pald1b s843Tg + MO1-tnfrsf1b s843Tg + MO1-tnfrsf21 s843Tg + MO1-tspan5b s843Tg + MO2-tnfrsf19 s843Tg + MO3-adcyap1r1a
Knockdown Reagents:	MO1-pald1b MO1-tnfrsf1b MO1-tnfrsf21 MO1-tspan5b MO2-tnfrsf19 MO3-adcyap1r1a
Anatomical Terms:	central artery blood vessel endothelium trunk vasculature blood vessel endothelium
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s843Tg + MO1-pald1b s843Tg + MO1-tnfrsf1b s843Tg + MO1-tnfrsf21 s843Tg + MO1-tspan5b s843Tg + MO2-tnfrsf19 s843Tg + MO3-adcyap1r1a
Knockdown Reagents:	MO1-pald1b MO1-tnfrsf1b MO1-tnfrsf21 MO1-tspan5b MO2-tnfrsf19 MO3-adcyap1r1a
Observed In:	angiogenesis brain vasculature central artery establishment of blood-brain barrier
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 22(2), Tam, S.J., Richmond, D.L., Kaminker, J.S., Modrusan, Z., Martin-McNulty, B., Cao, T.C., Weimer, R.M., Carano, R.A., van Bruggen, N., and Watts, R.J., Death Receptors DR6 and TROY Regulate Brain Vascular Development, 403-417, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell