FIGURE

Fig. 2

ID

ZDB-FIG-120126-35

Publication

Schepis et al., 2012 - αE-catenin regulates cell-cell adhesion and membrane blebbing during zebrafish epiboly

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Fig. 2

ctnna morphants are delayed in epiboly. (A-C) Immunofluorescence images of fixed zebrafish embryos stained for β-catenin. AP, animal pole; VP, vegetal pole. Upper arrow indicates deep cell margin, lower arrow indicates EVL margin. (D-F) Confocal fluorescence images of Ctnna antibody staining of the most external epiblast layer at the animal pole of embryos at 50% epiboly. Scale bar: 10 μm. (G) Rescue embryos stained for the HA tag; same view as D-F. Scale bar: 10 μm. (H) Percentage of epiboly of the deep cell (DC) and EVL margins. Three independent experiments, n=25 embryos. ^*P<0.006, ^#P<0.001. Error bars indicate s.d.

Expression Data

Gene:	ctnna1
Fish:	WT WT + MO1-ctnna1 WT + MO3-cdh1
Knockdown Reagents:	MO1-ctnna1 MO3-cdh1
Anatomical Terms:	DEL DEL cytoplasm DEL plasma membrane
Stage:	50%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ctnna1 WT + MO3-cdh1
Knockdown Reagents:	MO1-ctnna1 MO3-cdh1
Observed In:	DEL DEL cytoplasm DEL plasma membrane embryo development ending in birth or egg hatching epiboly involved in gastrulation with mouth forming second protein localization to plasma membrane
Stage Range:	50%-epiboly to 75%-epiboly

Phenotype Detail

Acknowledgments

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