FIGURE

Fig. 8

ID

ZDB-FIG-120126-41

Publication

Schepis et al., 2012 - αE-catenin regulates cell-cell adhesion and membrane blebbing during zebrafish epiboly

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Fig. 8

Blebbing triggered by αE-catenin depletion is E-cadherin dependent. (A) Selected confocal images from time-lapse movies of transplanted ctnna morphant cells expressing mbGFP in the most external epiblast external layer of the host cdh1 morphant embryos at 50% epiboly. Scale bar: 5 μm. (B) Confocal images from time-lapse movies at the most external epiblast layer of the animal pole of live embryos at 50% epiboly. Membranes are labeled with mbGFP. Arrows indicate membrane blebs. Embryos were co-injected with cdh1 and ctnna mo. Scale bar: 8 μm. (C) Percentage of cells exhibiting bleb-like protrusions. n=12 embryos. ^*P<10^–5. Error bars indicate s.e.m. (D) Average number of blebs per cell. n=12 embryos. ^*P<10^–8. Error bars indicate s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ctnna1 WT + MO1-ctnna1 + MO3-cdh1
Knockdown Reagents:	MO1-ctnna1 MO3-cdh1
Observed In:	DEL plasma membrane
Stage:	50%-epiboly

Phenotype Detail

Acknowledgments

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