FIGURE

Fig. 9

ID

ZDB-FIG-120126-42

Publication

Schepis et al., 2012 - αE-catenin regulates cell-cell adhesion and membrane blebbing during zebrafish epiboly

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Fig. 9

αE-catenin and E-cadherin depletion influence ERM protein localization and membrane blebbing in ezr morphants. (A) Percentage of cells with bleb-like membrane protrusions. n=12 embryos. ^*P<10^–6 ezr or ctnna mo low dose versus ctnna/ezr mo low dose, ^#P<10^–6 ezr mo high dose versus cdh1/ezr mo high dose. (B) Average number of blebs per cell. n=12 embryos. ^*P<10^–6 ezr or ctnna mo low dose versus ctnna/ezr mo low dose, ^#P<10^–5 ezr mo high dose versus cdh1/ezr mo high dose. Error bars indicate s.e.m. (C-E) Confocal images of the most external epiblast layer at the animal pole of embryos at 50% epiboly stained for phospho-ERM proteins. (C) Uninjected control. (D) ctnna morphant. (E) cdh1 morphant. Scale bar: <10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ezrb WT + MO1-ctnna1 + MO1-ezrb WT + MO1-ezrb + MO3-cdh1
Knockdown Reagents:	MO1-ctnna1 MO1-ezrb MO3-cdh1
Observed In:	DEL plasma membrane
Stage:	50%-epiboly

Phenotype Detail

Acknowledgments

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