FIGURE

Fig. 5

ID

ZDB-FIG-111128-38

Publication

Gallagher et al., 2011 - Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions

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Fig. 5

Rbfox1l/rbfox2 knockdown alters myofibril organization. (A–D) Anti-MHC immunohistochemistry at 24 and 48 hpf reveals that slow muscle fibers in rbfox1l/rbfox2 morphants are disorganized compared to normally striated fibers in wildtype muscle (A, C vs. B, D). (E–J) Immunohistochemistry with Alexa-Fluor 488-conjugated phalloidin, which labels F-actin, reveals that slow and fast muscle thin filaments are elongated and highly organized in wildtype embryos at 24 and 48 hpf, respectively (E, G), whereas thin filament structure is severely disrupted in Rbfox1l/rbfox2 morphants (F, H). Defects in myofiber organization (I–J) are less severe at lower rbfox1l/rbfox2 MO doses (1.5 ng each) when compared to higher doses (7 ng each). Doses in B, D, F, and H = 4.5 ng each MO. Scale bars = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-rbfox1l + MO1-rbfox2
Knockdown Reagents:	MO1-rbfox1l MO1-rbfox2
Observed In:	muscle myofibril muscle striated muscle thin filament
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 359(2), Gallagher, T.L., Arribere, J.A., Geurts, P.A., Exner, C.R., McDonald, K.L., Dill, K.K., Marr, H.L., Adkar, S.S., Garnett, A.T., Amacher, S.L., and Conboy, J.G., Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions, 251-61, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.