FIGURE

Fig. 4

ID

ZDB-FIG-111128-37

Publication

Gallagher et al., 2011 - Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Rbfox1l/Rbfox2 knockdown results in cardiac and skeletal muscle defects. (A, B) In Rbfox double morphants, myotomes are chevron-shaped as in control embryos (lateral view, one myotome is false-colored in each panel). (C–H) Polarized light microscopy reveals that rbfox1l/rbfox2 morphant muscle has delayed birefringence at 30 hpf (compare C with D) that partially recovers by 48 hpf (compare E with F). (G–I) Rbfox1l/Rbfox2 knockdown in myl7-GFP embryos that have GFP + myocardial cells, reveals cardiac muscle defects, with low rbfox1l/rbfox2 MO doses (1.5 ng each) causing ventricular defects and high doses (9 ng each) affecting the morphology of the entire heart. WT = mock-injected; MO = morpholino; a = atrium; v = ventricle. Scale bars = 100 μm (A–B; G–I), 500 μm (C–F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-rbfox1l + MO1-rbfox2 f1Tg + MO1-rbfox1l + MO1-rbfox2
Knockdown Reagents:	MO1-rbfox1l MO1-rbfox2
Observed In:	heart muscle myocardium ventricular cardiac muscle tissue morphogenesis
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 359(2), Gallagher, T.L., Arribere, J.A., Geurts, P.A., Exner, C.R., McDonald, K.L., Dill, K.K., Marr, H.L., Adkar, S.S., Garnett, A.T., Amacher, S.L., and Conboy, J.G., Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions, 251-61, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.