Fig. S11

Cre expression is restricted to radial glia in the adult zebrafish telencephalon in the her4.1:mCherryT2ACreER^T2 line. (A) In the adult telencephalon of transgenic (her4.1:mCherryT2ACreER^T2) fish, the expression of mCherry (red) is restricted to cells in the VZ that co-express the glial marker glutamine synthetase (GS, green, arrowheads), identifying them as radial glia. (B) To identify the cell type in which tamoxifen-dependent recombination occurs at the initial stages of lineage-tracing experiments analysis for the glial marker S100B and GFP were carried out using double transgenic her4.1:mCherryT2ACreER^T2 × hsp70:DsRed2(floxed)EGFP fish. Recombination was induced by five tamoxifen soakings and fish were analyzed 10 days after the first soaking. GFP expression (green) is restricted to the VZ and all cells also express S100B (red, arrowheads). Thus, tamoxifen-dependent recombination is restricted to radial glia in the adult zebrafish telencephalon. However, we also occasionally find S100B^-/GFP⁺ cells in the periventricular zone (one or two cells per section, data not shown). These cells are probably radial glia derived neurons that were generated in the 10 days prior to analysis. Scale bars: 100 μm in overviews, 10 μm in insets. v, ventricle.