Fig. 4
- ID
- ZDB-FIG-110622-27
- Publication
- Waxman et al., 2011 - Zebrafish retinoic acid receptors function as context-dependent transcriptional activators
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RAR-vps require RA to function. All images depict Tg(12XRARE-ef1a:gfp) embryos. (A, F) Uninjected control embryos. (B–E, K–M) Injection with (B) rarab-Nvp, (C) rarab-ΔAvp, (D) rarab-Cvp, (E) rarab-ΔFvp, (K) rarga-Nvp, (L) rarga-ΔAvp, or (M) rarga-ΔFvp mRNA induces ectopic reporter expression. (F–P) Treatment with 1 μM DEAB beginning at 40% epiboly blocks reporter expression in injected embryos. All images are dorsal views with anterior up. (Q) In HEK 293 cells transfected with the pGL3-basic:12XRARE-tk reporter, co-transfection with RAR-vp constructs caused similar trends of reporter activation to those found in zebrafish embryos. Arbitrary Units (AU) represents the ratio of firefly luciferase to Renilla luciferase. Each column represents the average AU plus standard deviation (bars) of 3 replicates for a condition from one representative experiment. Equivalent trends were observed using the pGL3-basic:12XRAR-ef1a reporter; additionally, DEAB treatment of cells transfected with RAR-vp constructs reduced reporter activation (JSW and DY, data not shown). |
Reprinted from Developmental Biology, 352(1), Waxman, J.S., and Yelon, D., Zebrafish retinoic acid receptors function as context-dependent transcriptional activators, 128-140, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.