FIGURE

Fig. s3

ID

ZDB-FIG-110510-8

Publication

Lobbardi et al., 2011 - Fine-tuning of Hh signaling by the RNA-binding protein Quaking to control muscle development

Other Figures

All Figure Page

Back to All Figure Page

Fig. s3

The qkA^gt-MO induces an alternative splicing site. (A) RT-PCR on uninjected or qkA^gt-MO-injected embryos. Two bands corresponding to the two qkA isoforms are amplified. A shift of both isoforms is seen in morphants. (B) Sequencing of each PCR-amplified isoform after gel extraction. With qkA^gt-MO, a cryptic splicing site is revealed that allows a short part of intron 1 to be transcribed. This induces a frameshift with the appearance of a stop codon in the intron 1 sequence (blue) interrupting qkA1 or qkA2 mRNA translation. L, 1 kb DNA ladder: lower band, 1 kb; upper band, 1.5 kb.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development