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Fig. s3
The qkAgt-MO induces an alternative splicing site. (A) RT-PCR on uninjected or qkAgt-MO-injected embryos. Two bands corresponding to the two qkA isoforms are amplified. A shift of both isoforms is seen in morphants. (B) Sequencing of each PCR-amplified isoform after gel extraction. With qkAgt-MO, a cryptic splicing site is revealed that allows a short part of intron 1 to be transcribed. This induces a frameshift with the appearance of a stop codon in the intron 1 sequence (blue) interrupting qkA1 or qkA2 mRNA translation. L, 1 kb DNA ladder: lower band, 1 kb; upper band, 1.5 kb.
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