FIGURE

Fig. 4

ID

ZDB-FIG-110510-4

Publication

Lobbardi et al., 2011 - Fine-tuning of Hh signaling by the RNA-binding protein Quaking to control muscle development

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Fig. 4

Convergence of qkA and Hh phenotypes. (A-F) qkA^t and gli2a^MO zebrafish embryos exhibit downregulated Hh signaling. Embryos were processed at the 10-somite stage for in situ hybridization with the indicated probes. Dorsal view. (G,H) Lateral views of Pax7-immunostained embryos at 24 hpf. Two populations of Pax7-positive cells can be discerned, of which dermomyotomal cells (arrowheads) exhibit the weaker expression. The number of dermomyotomal cells per somite is indicated beneath each somite. (I-Q) Slow fiber distal migration requires QkA and Hh activities. Embryos were stained at 24-hpf for slow fibers (F59) and all differentiated muscle fibers (MF20) as indicated, then analyzed by confocal microscopy (transverse sections). Note the presence of gaps (asterisks) and deep slow muscle cells (arrows) medial to the fast muscle cells in qkA^t mutants and gli2a morphants.

Expression Data

Genes:	prdm1a ptch2
Antibodies:	Ab-F59 Ab-MF20 Ab-Pax7
Fish:	WT qkia^{t31954/t31954} WT + MO2-gli2a
Knockdown Reagent:	MO2-gli2a
Anatomical Terms:	adaxial cell dermomyotome fast muscle cell slow muscle cell
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	qkia^{t31954/t31954} WT + MO2-gli2a
Knockdown Reagent:	MO2-gli2a
Observed In:	adaxial cell dermomyotome myotome skeletal muscle tissue development slow muscle cell smoothened signaling pathway
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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