FIGURE

Fig. 6

ID

ZDB-FIG-110510-6

Publication

Lobbardi et al., 2011 - Fine-tuning of Hh signaling by the RNA-binding protein Quaking to control muscle development

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Fig. 6

Analysis of QkA requirement for gli2a mRNA stability. (A) The region of the zebrafish gli2a 32UTR mRNA that contains the Quaking response element (QRE). Consensus sequences are in red. (B,C) Biosensor assay for QkA activity. RNA encoding d2eGFP flanked by the gli2a 32UTR or a control (Ctr) 32UTR were injected, together with kaede RNA as a control, into WT (+/+) or qkA^t mutants (-/-). (B) Proteins from each embryo were extracted at the 10-somite stage and probed by western blot. (C) Protein expression values from B were normalized to controls (see Materials and methods) and are plotted according to the genotype. Error bars indicate s.d. (D-F) Slow muscle fiber staining in yot^ty119 embryos injected with qkA^ATG-MO or gli2a^MO.

Expression Data

Antibody:	Ab-F59
Fish:	gli2a^ty119/ty119 gli2a^ty119/ty119 + MO2-gli2a gli2a^ty119/ty119 + MO1-qkia
Knockdown Reagents:	MO1-qkia MO2-gli2a
Anatomical Term:	slow muscle cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gli2a^ty119/ty119 gli2a^ty119/ty119 + MO2-gli2a gli2a^ty119/ty119 + MO1-qkia
Knockdown Reagents:	MO1-qkia MO2-gli2a
Observed In:	3'-UTR-mediated mRNA stabilization muscle cell differentiation muscle pioneer slow muscle cell smoothened signaling pathway somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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