FIGURE

Fig. S2

ID

ZDB-FIG-110128-12

Publication

Haud et al., 2011 - rnaset2 mutant zebrafish model familial cystic leukoencephalopathy and reveal a role for RNase T2 in degrading ribosomal RNA

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Fig. S2

Confirmation of the AO127 mutation by knockdown. (A) Schematic of the zebrafish rnaset2 gene mutated in AO127 mutants showing exon–intron structure and the position of a MO designed to interrupt splicing of coding exon 3. (B) RT-PCR revealed the presence of mRNA retaining intron 3 from animals injected with the rnaset2 MO. Residual WT mRNA is presumably maternal. β-Actin serves as a control for RNA integrity and equal input cDNA. (C) Injection of rnaset2 MO, but not control MO, in animals heterozygous for rnaset2 resulted in an increase in AO staining.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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