FIGURE

Fig. 8

ID

ZDB-FIG-110111-19

Publication

Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation

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Fig. 8

Analysis of transformed cell clones in larvae where immune cell interaction is disrupted.

(A–C) Typical confocal Z-projections of 3-dpf V12RAS⁺ larvae that were previously injected with (A) control (n = 26), (B) pu.1 (n = 31), and (C) DUOX (n = 14) morpholinos. (D) Quantification of the numbers of V12RAS⁺ cells in equivalent flank skin within these three groups at 3 dpf. (E and F) Typical confocal Z-projection images of control and DPI-treated larvae at 60 hpf for similar analysis of V12RAS⁺ cells. (G) Quantification of V12RAS⁺ cell numbers in control (n = 10) versus DPI-treated (n = 12) larvae at 60 hpf; *, p<0.05; ***, p<0.001. (H) Graphic representation of BrdU incorporation by V12RAS⁺ cells in pu.1 morphant (n = 33) versus control (n = 21) larvae. Each point on the scatter plots represents one larva. Scale bar = 150 μm.

Expression Data

Gene:	EGFP
Fish:	io006Tg; zf391Tg io006Tg; zf391Tg + MO1-duox io006Tg; zf391Tg + MO1-spi1b
Knockdown Reagents:	MO1-duox MO1-spi1b
Anatomical Term:	motile cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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