Fig. 2

Comparison of leukocyte recruitment to V12RAS⁺ transformed cells versus to a wound.

(A) Confocal image of the trunk epidermis (region indicated by schematic) from a movie of a control Tg(kita:GalTA4, UAS:eGFP, LysC:DsRed) larva (Video S3A). (B) DIC image of a skin laser wound in the same region of the trunk as the transformed cells in (C). Inset is a confocal image of the wound highlighting how LysC:DsRed⁺ cells are recruited to the wound center (Video S3B). (C) Equivalent image to (A) but from a Tg(kita:GalTA4, UAS:V12RASeGFP, LysC:DsRed) larva (Video S3C). (D–F) Superimposition of DsRed⁺ cell profiles (orange) from all time frames of 80-min time-lapse movies of (D) control, (E) wounded, and (F) V12RAS⁺ larvae, to reveal cumulative footprints (orange). (G–I) as for (D–F) but with tracks of all the LysC:DsRed⁺ cells that migrated within the field of view of the movies superimposed onto a single movie still. (J) Quantification of the numbers of LysC:DsRed⁺ cells that migrate through the imaging fields of control, V12RAS⁺, and wounded larvae. (K) As for (J) but quantification of the cell MI for each of these conditions. **, p<0.01; ***, p<0.001. Scale bars = 48 μm for all the images. In all images larvae are 4-dpf.