FIGURE

Fig. 7

ID

ZDB-FIG-110111-18

Publication

Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Analysis of leukocyte recruitment in chimeric V12RAS-transformed cell-bearing larvae.

(A) Schematic diagram to illustrate how we generate chimeric V12RAS⁺ transformed cell-bearing Tg(LysC:DsRed) larvae, in which either the donor transformed cells or host tissues are morphant for DUOX. (B) A still image from Video S13B illustrating how LysC:DsRed⁺ cells are recruited to DUOX morphant V12RAS⁺ cells in WT host environment. (C) A still image from Video S13C showing LysC:DsRed⁺ cells recruited to WT V12RAS⁺ cells in a DUOX morphant host environment. (D–F) Cumulative LysC:DsRed⁺ cell footprints (orange) of representative movies shown in Video S13. (G) Graph to illustrate numbers of recruited LysC⁺ cells drawn towards V12RAS⁺ transformed cells in the two transplantation scenarios above compared with pTol2-hsp:V12RASeGFP injected into Tg(LysC:DsRed) control. **, p<0.01; ***, p<0.001; ns, not significant. Scale bars = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.