FIGURE

Fig. 5

ID

ZDB-FIG-101118-5

Publication

Chitramuthu et al., 2010 - Progranulin modulates zebrafish motoneuron development in vivo and rescues truncation defects associated with knockdown of Survival motor neuron 1

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Fig. 5

Progranulin modulates motoneuron development in vivo: zfPGRN-A knockdown induced CaP MN defects that were partially rescued by either zfPGRN-A or hPGRN mRNA. Lateral views (anterior to the left; dorsal to the top) of embryos labelled with znp1 mAb at 27 hpf in (A) wild type embryos, (B) embryos injected with Control MO, (C) 100 pg zfPGRN-A mRNA, (D) 100 pg hPGRN mRNA, (E) zfPGRN-A MO1, (F) zfPGRN-A MO2, (G) zfPGRN-A MO2+100 pg zfPGRN-A mRNA and (H) zfPGRN-A MO2+100 pg hPGRN mRNA. Observed phenotypes were normal MN development (A, B), increase in branched axons (C-H), truncation of axons (E, F) and partial rescue of truncated MNs (G, H). Dashed lines represent horizontal myoseptum. Abbreviations: T-truncation; B-Branching. Scale Bar = 50 μm.

Expression Data

Antibody:	znp-1
Fish:	WT WT + MO3-grna WT + MO4-grna
Knockdown Reagents:	MO3-grna MO4-grna
Anatomical Term:	CaP motoneuron axon
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-grna WT + MO4-grna
Knockdown Reagents:	MO3-grna MO4-grna
Observed In:	axon extension CaP motoneuron axon
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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