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Fig. 5 Progranulin modulates motoneuron development in vivo: zfPGRN-A knockdown induced CaP MN defects that were partially rescued by either zfPGRN-A or hPGRN mRNA. Lateral views (anterior to the left; dorsal to the top) of embryos labelled with znp1 mAb at 27 hpf in (A) wild type embryos, (B) embryos injected with Control MO, (C) 100 pg zfPGRN-A mRNA, (D) 100 pg hPGRN mRNA, (E) zfPGRN-A MO1, (F) zfPGRN-A MO2, (G) zfPGRN-A MO2+100 pg zfPGRN-A mRNA and (H) zfPGRN-A MO2+100 pg hPGRN mRNA. Observed phenotypes were normal MN development (A, B), increase in branched axons (C-H), truncation of axons (E, F) and partial rescue of truncated MNs (G, H). Dashed lines represent horizontal myoseptum. Abbreviations: T-truncation; B-Branching. Scale Bar = 50 μm.