FIGURE

Fig. 1

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ZDB-FIG-101011-46

Publication

Rai et al., 2010 - DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC

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Fig. 1

Upregulation of DNA Demethylase Components, and Hypomethylated Genes in apc Mutant Embryos and FAP Adenomas

(A–D) Whole-mount in situ staining for aid (A), apobec2a (A), apobec2b (A), mbd4 (B), tdg (B), gadd45α (C), gadd45βlike (C), gadd45β (C), gadd45γ (C), and dnmt1 (D) in apc mutants (apc^mcr) and siblings (apc^wt) zebrafish embryos at 72 hpf.

(E) MeDIP-qPCR for genes shown (selected from genome-wide MeDIP-ChIP microarray analysis) in apc^mcr and apc^wt (72 hpf) that are either uninjected or injected with aaa Mo (combination of aid, apobec2a, and apobec2b morpholinos; 0.5 ng each) or mbd4 and tdg morpholinos together (1 ng each) or V5-Dnmt1 expressing plasmid (1 pg, an amount that rescues Dnmt1 morphants with 97% knockdown of Dnmt1 levels). Dnmt1 protein levels are in Figure S1C.

(F) MeDIP-qPCR for genes shown (selected from genome-wide MeDIP-ChIP microarray analysis) in human adenomas (A) and matching uninvolved tissues (U) from FAP patients. P1-P10 refers to ten different patients.

In both (E) and (F), the y axis shows values for each promoter region normalized to a negative control region lacking CpGs and then normalized to the values from wild-type or uninvolved, valued at 1.

Error bars indicate ± standard deviation (SD). See also Figure S1.

Expression Data

Genes:	aicda apobec2a apobec2b dnmt1 gadd45aa gadd45ab gadd45ba gadd45ga mbd4
Fish:	WT apc^hu745/hu745
Anatomical Terms:	head intestine whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apc^hu745/hu745
Observed In:	digestive tract mesoderm development intestine
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 142(6), Rai, K., Sarkar, S., Broadbent, T.J., Voas, M., Grossmann, K.F., Nadauld, L.D., Dehghanizadeh, S., Hagos, F.T., Li, Y., Toth, R.K., Chidester, S., Bahr, T.M., Johnson, W.E., Sklow, B., Burt, R., Cairns, B.R., and Jones, D.A., DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC, 930-942, Copyright (2010) with permission from Elsevier. Full text @ Cell