FIGURE

Fig. 2

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ZDB-FIG-101011-47

Publication

Rai et al., 2010 - DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC

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Fig. 2

APC Negatively Regulates Demethylase Expression via Retinoic Acid

(A) Whole-mount in situ staining for aid, gadd457alpha;, and mbd4 in apc^mcr and apc^wt embryos treated with DMSO, all-trans retinoic acid (ATRA, 1 μM), or Cox-2 inhibitor NS-398 (10 μM) or injected with control morpholino or ctbp1 morpholino (2 ng). β-catenin knockdown efficiency with NS-398 treatment is depicted in Figure S2A.

(B and C) Quantitative RT-PCR for aid, mbd4, and gadd45α in apc^mcr and apc^wt embryos treated with DMSO or ATRA (B) or in different RA deficiency models in zebrafish, as shown in (C). Expression of genes are first normalized to 28S and then to the control embryo mRNA/28S ratio, valued as 1.

(D) MeDIP-qPCR for genes shown (as in Figure 1E) in apc mutants (72 hpf) that are either untreated or treated with ATRA.

Error bars indicate ± SD. See also Figure S2.

Expression Data

Genes:	aicda gadd45ab mbd4
Fish:	WT apc^hu745/hu745 apc^hu745/hu745 + MO1-ctbp1
Condition:	chemical treatment: all-trans-retinoic acid
Knockdown Reagent:	MO1-ctbp1
Anatomical Terms:	head intestine trunk
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	apc^hu745/hu745 apc^hu745/hu745 + MO1-ctbp1
Knockdown Reagent:	MO1-ctbp1
Observed In:	intestine obsolete DNA demethylation obsolete regulation of DNA demethylation retinoic acid receptor signaling pathway
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 142(6), Rai, K., Sarkar, S., Broadbent, T.J., Voas, M., Grossmann, K.F., Nadauld, L.D., Dehghanizadeh, S., Hagos, F.T., Li, Y., Toth, R.K., Chidester, S., Bahr, T.M., Johnson, W.E., Sklow, B., Burt, R., Cairns, B.R., and Jones, D.A., DNA demethylase activity maintains intestinal cells in an undifferentiated state following loss of APC, 930-942, Copyright (2010) with permission from Elsevier. Full text @ Cell